A Combined Colon Organoid-Sensory Neuron Model Reveals Epithelial Contribution to Moringin Efficacy Against Painful Inflammatory Bowel Disease - PubMed
5 hours ago
- #Moringa oleifera
- #IBD
- #visceral pain
- Visceral pain is a major symptom of inflammatory bowel diseases (IBDs), requiring effective treatment strategies.
- Moringa oleifera constituents, particularly moringin (MOR), show beneficial properties against inflammation-induced epithelial dysfunction and visceral hypersensitivity.
- A three-stage experimental strategy was used: murine colon organoids exposed to pro-inflammatory cytokines, epithelial-neuronal communication assessment, and in vivo validation in a DSS-induced colitis model.
- MOR 30 μM co-treatment normalized oxidative stress and gene expression in inflamed organoids but did not affect cytotoxicity.
- Conditioned media from inflamed organoids increased c-Fos and CGRP expression in DRG neurons, which was prevented by MOR treatment.
- Oral administration of MYR-bioactivated GMG (GMG + MYR) in DSS-treated mice reduced visceral hypersensitivity, pain-associated behaviors, and colonic damage in a dose-dependent manner.
- MOR/GMG + MYR demonstrated gut-protective and analgesic effects under intestinal inflammation.
- Intestinal organoids effectively model inflammation and therapeutic responses, potentially reducing animal use in IBD research.