DNA/RNA-Based Next-Generation Sequencing (NGS) Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A Six-Year, Prospective, Multi-Institutional, Real-Time Study - PubMed
5 hours ago
- #Next-Generation Sequencing
- #Biliary Stricture
- #Early Diagnosis
- DNA/RNA-based NGS (BiliSeqV2/V3) improves diagnosis of neoplastic bile duct strictures with 82% sensitivity and 98% specificity.
- Pathologic assessment alone has lower sensitivity (44%) but high specificity (99%).
- Combining NGS with pathologic assessment increases sensitivity to 88% while maintaining 97% specificity.
- NGS shows higher sensitivity in high-risk groups (Hispanic, germline carriers, PSC patients) compared to pathology.
- Actionable molecular alterations were found in 20% of BiliSeqV3-positive neoplasms, altering management in 30% of cases.