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Innate lymphoid cell heterogeneity and etiology-specific reprogramming in hepatocellular carcinoma - PubMed

3 months ago
  • #Innate lymphoid cells
  • #Tumor microenvironment
  • #Hepatocellular carcinoma
  • Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths globally.
  • The study profiles innate lymphoid cells (ILCs) in HCC using scRNA-seq, bulk RNA-seq, and CyTOF from 50 patients with different etiologies (HBV and non-viral).
  • ILC1 subsets were identified as proliferative (ILC1p) and cytotoxic (ILC1c), while ILC2 showed type-2 immune traits and ILC3 expressed key transcription factors and IL18.
  • ILC subsets varied significantly by disease etiology, with NV-HCC ILC2s showing pro-fibrotic and tumor-promoting signatures.
  • ILC1s in NV-HCC exhibited activated and cytotoxic phenotypes, whereas HBV-HCC ILC1s showed exhaustion markers like CD96 and TIGIT.
  • NV-HCC ILC1s demonstrated enhanced IL-2/IL-15 signaling and interactions with CD8+ T cells via HLA-E, suggesting antitumor potential.
  • The study highlights etiology-associated differences in ILC phenotypes and their roles in modulating immune responses in HCC.