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O-GlcNAcylation of YAP1 promotes lung transplant ischemia-reperfusion injury via binding to HIF1α transcription factor and activating autophagy and mitophagy - PubMed

5 hours ago
  • #Autophagy
  • #Ischemia-Reperfusion Injury
  • #YAP1
  • O-GlcNAcylation of YAP1 exacerbates lung transplant ischemia-reperfusion injury.
  • Hypoxia-reoxygenation activates the Hippo-YAP1 pathway, increasing lung epithelial cell damage.
  • Autophagy and mitophagy levels rise significantly during ischemia-reperfusion, indicating cellular stress.
  • YAP1 knockdown reduces autophagy and mitophagy under hypoxia-reoxygenation and ischemia-reperfusion conditions.
  • OGT-mediated O-GlcNAc modification of YAP1 enhances its interaction with HIF1α, activating hypoxia-responsive molecules.
  • Knockdown of OGT mitigates autophagy, mitophagy, and associated damage in lung epithelial cells and transplant tissues.
  • The study suggests potential therapeutic targets for reducing lung transplant ischemia-reperfusion injury.