RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion - PubMed
5 hours ago
- #nuclear pore complexes
- #RanBP2
- #annulate lamellae
- Nuclear pore complexes (NPCs) facilitate nucleocytoplasmic transport and are found in the nuclear envelope (NE) and annulate lamellae (AL).
- AL, previously thought to be exclusive to embryonic and malignant cells, are also present in somatic mammalian cells.
- Under normal conditions, AL store pre-assembled AL-NPCs that integrate into the NE, contributing to about one-third of new nuclear pores and supporting nuclear expansion during G1 phase.
- Pathological stimuli impair AL transfer to the NE, leading to their cytoplasmic accumulation.
- RanBP2 (Nup358) is crucial for AL biogenesis, with its phenylalanine-glycine repeats promoting AL-NPC scaffold oligomerization.
- ER-associated Climp63 (CKAP4) directs AL-NPCs to ER sheets and the NE.
- AL-driven nuclear pore formation is a distinct pathway complementary to canonical NPC assembly routes.