OMIP-120: A 22-Marker Spectral Flow Cytometry Panel for the Characterization of Major Immune Populations in Murine Bone Marrow and Osteosarcoma Tissue - PubMed
5 hours ago
- #spectral flow cytometry
- #osteosarcoma immune microenvironment
- #bone marrow immunology
- Developed a 22-marker spectral flow cytometry panel for high-dimensional immune profiling in murine bone marrow and osteosarcoma tissue.
- The panel enables characterization of major immune populations including T cells, B cells, NK cells, dendritic cells, monocytes, macrophages, and neutrophils.
- Includes activation, exhaustion, and immune checkpoint markers to study tumor-induced immune remodeling and immune evasion in the bone marrow microenvironment.
- Optimized using spleen, bone marrow, and osteosarcoma tissue from BALB/c mice, applicable for preclinical models of bone and bone marrow cancers.
- Provides an adaptable platform for immune profiling across solid tumors and hematological malignancies to improve understanding of immune suppression in bone tumors.