CRISPR/Cas9 screening with destabilized bicistronic fluorescent protein reporter revealed PABPN1 as a hub of regulators for alternative polyadenylation - PubMed
5 hours ago
- #PABPN1
- #Polyadenylation
- #CRISPR
- CRISPR/Cas9 screening with a destabilized bicistronic fluorescent protein reporter (dBFPR) was used to identify regulators of alternative polyadenylation (APA).
- The study found that PTBP1, ELAVL1, and DDX3X regulate APA and promote cell proliferation by interacting with PABPN1.
- PABPN1 was identified as a central hub for APA regulation.
- A high-throughput screening platform combining CRISPR/Cas9, dBFPR, and fluorescence-activated cell sorting was developed for this research.