VAMP8 function reveals tight linkage between endocytic recycling and endocytosis - PubMed
5 hours ago
- #recycling
- #endocytosis
- #VAMP8
- VAMP8 knockdown impairs multiple stages of clathrin-mediated endocytosis (CME), including initiation, stabilization, and invagination of clathrin-coated pits.
- VAMP8's role in CME is indirect; it does not need to be recruited to clathrin-coated vesicles for CME progression.
- Depletion of VAMP8 inhibits recycling of endocytic cargoes, such as transferrin receptor, redirecting them toward lysosomal degradation.
- This inhibition of recycling leads to endocytic cargo depletion, which indirectly impairs clathrin-coated vesicle formation and inhibits CME.
- The study highlights the critical role of cargo recruitment in stabilizing nascent clathrin-coated pits and underscores the crosstalk between endocytosis and endocytic recycling.