Single-cell spatial transcriptomics reveals tumor microenvironment heterogeneity in primary and lymph node-metastatic small cell lung cancer - PubMed
6 hours ago
- #lymph node metastasis
- #spatial transcriptomics
- #tumor microenvironment
- Single-cell spatial transcriptomics study of 105 primary and lymph node metastatic specimens from 75 small cell lung cancer (SCLC) patients.
- Identified three lymph node metastasis (LNM)-enriched malignant subclusters with distinct metabolic and angiogenic programs linked to immune exclusion.
- Revealed vascular-immune crosstalk with endothelial cells orchestrating immune activation by avoiding malignant cells and forming perivascular niches with cytotoxic T cells.
- Cellular neighborhood analysis identified multicellular niches and a pan-immune hotspot (PIHs-1), whose abundance independently predicts survival.
- Provides a high-resolution spatial map of SCLC tumor microenvironment during LNM, offering mechanistic insights and potential biomarkers.