Hydrogen gas (H2) pretreatment improves lipopolysaccharide-induced acute liver injury in mice by inhibiting NLRP3 inflammasome activation and pyroptosis signaling - PubMed
6 hours ago
- #acute liver injury
- #NLRP3 inflammasome
- #hydrogen gas therapy
- Hydrogen gas (H2) pretreatment via intraperitoneal injection improves acute liver injury induced by lipopolysaccharide (LPS) in mice.
- H2 reduces inflammatory cell infiltration, lowers serum ALT and AST levels, and modulates oxidative stress markers (decreases 3-nitrotyrosine, MDA, MPO; increases GSH).
- Mechanistically, H2 inhibits TLR4-mediated signaling pathways (IKK-NF-κB and MAPK), leading to reduced pro-inflammatory cytokines (TNF-α, IL-1β, IL-18).
- H2 suppresses NLRP3 inflammasome activation (NLRP3, ASC, Caspase-1) and pyroptosis signaling involving GSDMD, Caspase-8/11, Caspase-3, and GSDME, as shown by reduced TUNEL staining.
- Pharmacological experiments using NLRP3 agonist (nigericin) and antagonist (MCC950) confirm H2's action on NLRP3 in vitro.
- H2 alleviates LPS-induced acute liver injury by modulating redox homeostasis, innate immune signaling, NLRP3 inflammasome, and pyroptosis.