Machine learning reveals targets of Gnaphalium hypoleucum DC. flavonoids against rheumatoid arthritis through gut microbiota and anti-inflammation - PubMed
6 hours ago
- #gut microbiota
- #machine learning
- #rheumatoid arthritis
- Machine learning identified key targets of Gnaphalium hypoleucum DC. flavonoids (GHTFs) for rheumatoid arthritis (RA) treatment.
- GHTFs and amentoflavone (AF) showed anti-inflammatory effects by inhibiting IL-17, TNF-α, and NF-κB pathways in vitro.
- In vivo, GHTFs reduced arthritis scores by over 40%, lowered TNF-α and IL-1β levels, and improved gut microbiota balance.
- GHTFs enriched beneficial Lactobacillus species and reduced pro-inflammatory gut bacteria, aiding immune regulation.
- Molecular docking confirmed stable binding of AF to core targets like MMP9, MMP13, TOP2A, and ALOX5.