Advanced physiological maturation of human iPSC-derived cardiomyocytes using an algorithm-directed optimization of defined media components - PubMed
6 hours ago
- #stem cells
- #media optimization
- #cardiomyocytes
- The study presents a new maturation medium (C16) for hiPSC-derived cardiomyocytes, developed using a differential evolutionary algorithm to optimize metabolic function.
- Compared to existing high-performing media, the new formulation significantly improves morphology, calcium handling, electrophysiology, and metabolism in both pure and co-cultured microtissue formats.
- Multi-omic screening validates the advanced maturation, providing a reliable workflow for generating highly functional hiPSC-CMs for disease modeling and drug testing.
- The intellectual property for the C16 medium has been patented and licensed to a biotechnology company, with several authors declaring related competing interests.