Sensory Nerves Regulate Odontoblast Differentiation via the SPP1/ITGA4 Axis During Tooth Root Development - PubMed
3 days ago
- #tooth root development
- #sensory nerves
- #odontoblast differentiation
- Sensory nerves play a crucial role in tooth root development by regulating odontoblast differentiation via the SPP1/ITGA4 axis.
- The study utilized trigeminal ganglion-conditioned medium (TG-CM) and subrenal co-culture models to demonstrate sensory nerve function in odontoblastic differentiation and root elongation.
- Integrated scRNA-seq analysis identified sensory nerve-derived factors like SPP1, CGRP, and KITL, which are essential for tooth root development.
- CellChat analysis revealed SPP1-ITGA4 as a critical ligand-receptor interaction, confirmed by Co-IP and proximity ligation assays (PLA).
- Knockdown of Itga4 impaired odontoblastic differentiation and abolished SPP1-promoted odontogenesis, highlighting its importance in the process.
- The findings provide insights into neuro-mesenchymal crosstalk, offering potential for stem cell therapies and tooth root regeneration.