Substrate selectivity of the human RNA m5C methyltransferase NSUN2 - PubMed
2 hours ago
- #RNA methylation
- #NSUN2
- #epitranscriptomics
- NSUN2 is a key human RNA methyltransferase for 5-methylcytosine (m5C) modifications, with dysregulation linked to cancers and neurological disorders.
- The study reveals how NSUN2 interacts with RNA substrates during its catalytic cycle and identifies RNA duplexes surrounding the m5C site as crucial for recognition.
- A minimized substrate for NSUN2 is defined as a dual-stem structure containing the CNNRR motif at the 5' end of the first stem, highlighting the importance of RNA structure and sequence.
- Insights into NSUN2's substrate specificity offer potential for understanding and therapeutically targeting NSUN2-dependent methylation in diseases.