Fusogenic Liposome-Mediated Extracellular Vesicles MicroRNA Profiling for Lung Cancer Diagnosis - PubMed
6 hours ago
- #lung cancer diagnosis
- #extracellular vesicles
- #liquid biopsy
- A fusogenic liposome nanoreactor was developed for direct profiling of plasma extracellular vesicle-associated microRNAs (EVs-miRNAs) for lung cancer diagnosis.
- The platform integrates reagent-encapsulating liposomes, isothermal rolling circle amplification, and CRISPR-Cas12a endonuclease cleavage in a one-pot method, enabling ultrasensitive detection down to fM levels.
- A lung cancer-specific EVs-miRNA signature was identified through a four-phase screening procedure and validated using public databases and clinical samples.
- In a clinical cohort study (n=141), the signature achieved up to 93.1% accuracy for early diagnosis, therapy monitoring, and prognosis evaluation, streamlining the workflow into a single RNA extraction-free step.