Ca²⁺ leakage is a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repair - PubMed
4 hours ago
- #Ca2+ Leakage
- #Membrane Repair
- #Non-canonical LC3 Lipidation
- Ca²⁺ leakage is identified as a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repair in human macrophages.
- A newly developed Ca²⁺ probe was used to record Ca²⁺ leakage history, revealing its role in triggering ATG8/LC3 lipidation after sterile membrane damage.
- Damaged compartments included both single and multilayered membrane structures undergoing extensive remodeling, with their complexity and acidification dependent on the damage trigger.
- Non-canonical LC3 lipidation on damaged membranes promotes membrane repair independently of canonical autophagy and ESCRT components CHMP2A/CHMP4B.
- The study highlights the role of endolysosomal Ca²⁺ leakage in initiating membrane repair mechanisms in macrophages.