Whole-embryo spatial transcriptomics at subcellular resolution from gastrulation to organogenesis - PubMed
14 hours ago
- #embryogenesis
- #gene expression
- #spatial transcriptomics
- Introduction of whole-embryo imaging platform (weMERFISH) for spatial transcriptomics at subcellular resolution.
- Quantified expression of 495 genes in zebrafish embryos and created an online atlas with data on 25,872 genes and 294,954 chromatin regions.
- Found that expression patterns often match tissue-specific accessible elements and changes align with cellular maturation and morphogenesis.
- Integration with live imaging showed that similar expression patterns can emerge through different dynamics.
- Revealed that sharp boundaries in gene expression develop through changes in gene expression rather than cell sorting.