Single-Cell Sequencing and Transcriptomic Sequencing Exploration of the Mechanisms Governing Lactylation in Mitochondrial Diseases - PubMed
4 days ago
- #lactylation
- #mitochondrial diseases
- #single-cell sequencing
- Mitochondrial diseases (MDs) are disorders caused by mutations in nuclear or mitochondrial DNA, affecting multiple organ systems due to mitochondria's role in energy generation.
- The study explores the link between lactylation and MDs using single-cell RNA sequencing (scRNA-seq) and bulk transcriptomic analyses.
- CD4+ T cells showed the highest lactylation scores among immune cell clusters identified in the single-cell analysis.
- Machine learning identified three core genes (EIF3D, SOD1, RPS26) strongly associated with MD pathogenesis, validated in muscle tissue.
- These genes correlate with immune cell populations, suggesting roles in immune regulation and metabolic processes.
- Pathway analyses revealed signaling mechanisms contributing to MD development, offering potential therapeutic targets.