Multiplexed antigen panel analysis identifies B cell phenotype and receptor genetic contributions to antibody breadth - PubMed
6 hours ago
- #SARS-CoV-2 variants
- #vaccine development
- #B cell immunology
- A highly multiplexed antigen panel was used to analyze B cells from COVID-19 mRNA vaccinees, infected patients, and organ donors, identifying B cell subsets associated with variant binding breadth.
- Atypical B cells and a subset of class-switched memory B cells with recent germinal center exposure were enriched for antigen binding, with post-germinal center cells showing increased breadth and somatic hypermutation over time.
- mRNA vaccination preferentially stimulated B cells producing antibodies with inherently higher antigen-binding breadth compared to infection, highlighting key factors for vaccine design against rapidly mutating viruses.