Integrative Single-Cell and Spatial Transcriptomics Reveal Organelle Stress-Associated Heterogeneity and Immune Microenvironment Remodeling in Lung Adenocarcinoma - PubMed
3 days ago
- #Organelle Stress
- #Lung Adenocarcinoma
- #SLC16A14
- The study uses integrative single-cell and spatial transcriptomics to analyze organelle stress-associated heterogeneity and immune microenvironment remodeling in Lung Adenocarcinoma (LUAD).
- Transcriptomic profiles and clinical data from TCGA-LUAD and public cohorts were analyzed, focusing on organelle stress-related pathways to classify LUAD into two subtypes: MRB (mitochondrial-ribosome biogenesis) and LC (lysosomal catabolism).
- MRB subtype showed enhanced stress and metabolic adaptation with immune exclusion features, while LC subtype had a relatively immune-active tumor microenvironment, impacting immunotherapy potential.
- SLC16A14 (MCT14) emerged as a key molecule linking stress heterogeneity to malignant progression, validated through in vitro silencing experiments that suppressed tumor cell proliferation, invasion, and migration.
- The findings provide a subtyping and prognostic framework for LUAD, revealing stress adaptation's role in tumor microenvironment remodeling and suggesting SLC16A14 and its communication network as potential therapeutic targets.