TASP1-mediated cleavage of REV3L enhances the activity of DNA polymerase ζ in mammalian cells - PubMed
5 hours ago
- #REV3L cleavage
- #DNA polymerase regulation
- #DNA damage tolerance
- TASP1 cleaves REV3L to produce stable fragments (Nter-p70 and Cter-p300) that form a heterodimer with enhanced polymerase activity.
- Cleaved Polζ complex associates with chromatin, binds nascent DNA, and undergoes ATR-dependent phosphorylation under replication stress.
- Preventing REV3L cleavage impairs Polζ activity, reduces cisplatin-induced mutagenesis, and increases homologous recombination markers like RAD51 foci.