Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase - PubMed
6 hours ago
- #protein quality control
- #nascent chain interactors
- #co-translational E3 ubiquitin ligase
- Nascent polypeptide chains interact with co-translational factors during translation, crucial for protein biogenesis and quality control.
- Developed nascent-chain interactor profiling (NCIP), a proteomics method to identify proteins interacting with nascent chains proteome-wide.
- NCIP supports the co-translational assembly model of protein complexes and identifies TRIM25 as a co-translational E3 ubiquitin ligase.
- TRIM25 ubiquitinates misfolded nascent chains for quality control at the ribosome.
- Provides a framework to systematically profile co-translational interactors.