Concurrent Burkholderia cepacia detected by plasma mcfDNA sequencing in a child with MRSA liver abscess and chronic granulomatous disease: a case report - PubMed
4 hours ago
- #Chronic granulomatous disease
- #mcfDNA sequencing
- #Burkholderia cepacia
- An 11-month-old male with CGD presented with fever, irritability, and bulky cervical lymphadenopathy unresponsive to antibiotics, highlighting delayed diagnosis due to nonspecific features.
- Imaging revealed a hepatic abscess and splenic/hepatic granulomas; MRSA was cultured from liver drainage, but symptoms persisted despite vancomycin therapy, suggesting additional pathogens.
- Plasma mcfDNA sequencing detected concurrent Burkholderia cepacia infection, guiding treatment with trimethoprim-sulfamethoxazole, which led to rapid symptom resolution.
- Genetic and dihydrorhodamine flow cytometric testing confirmed CGD, emphasizing the need for concurrent infection consideration in immunocompromised patients with febrile illness non-responsive to therapy.
- Plasma mcfDNA-Seq is a promising non-invasive tool for pathogen detection in CGD, especially when conventional diagnostics fail, aiding in timely diagnosis and management.