Autophagosome turnover requires Arp2/3 complex-mediated maintenance of lysosomal integrity - PubMed
4 hours ago
- #lysosomal integrity
- #Arp2/3 complex
- #autophagy
- Autophagy is a crucial intracellular degradation process for homeostasis, stress response, and preventing aging and disease.
- The Arp2/3 complex, an essential actin nucleator, plays a key role in autophagosome turnover by maintaining lysosomal integrity.
- Inducible knockout (iKO) of the Arp2/3 complex in mouse fibroblasts led to increased autophagy receptors and lipidated LC3/GABARAP proteins.
- Arp2/3 complex deficiency resulted in abnormal autolysosome accumulation and impaired turnover due to lysosomal damage.
- WHAMM, an Arp2/3-activating protein, is recruited to lysosomes upon membrane disruption, and its deletion increases lysosomal damage.
- The study highlights the importance of the Arp2/3 complex and WHAMM in maintaining lysosomal integrity for effective autophagic degradation.