Systematic identification of variant-specific RNA structure-small molecule interactions exemplified by RNA G-quadruplexes - PubMed
5 hours ago
- #BIVID-MaP
- #RNA-small molecule interactions
- #G-quadruplex
- Introduction of BIVID-MaP, a high-throughput method for detecting RNA-small molecule interactions.
- BIVID-MaP combines binding-dependent covalent modification with deep sequencing to profile deletions upon reverse transcription.
- Discovery of variant-specific interactions between a G-quadruplex-binding small molecule and RNAs with single-nucleotide variants.
- Cancer-associated somatic mutations found to influence small molecule binding by affecting G4 RNA structures.
- Potential for BIVID-MaP to contribute to the development of RNA-targeting drugs by identifying mutation-specific interactions.