Development of an S protein-based indirect ELISA for detecting IgA antibodies against porcine deltacoronavirus - PubMed
9 hours ago
- #PDCoV
- #IgA
- #ELISA
- The study developed an indirect ELISA based on the PDCoV S protein to detect IgA antibodies against porcine deltacoronavirus.
- The PDCoV S protein was expressed in a monoclonal CHO cell line and used as the coating antigen for high specificity and sensitivity.
- The assay showed no cross-reactivity with other swine pathogens, and demonstrated high concordance with IFA results for serum and milk samples.
- This iELISA is a reliable tool for PDCoV IgA detection in serum and milk, useful for infection surveillance and vaccine evaluation.