Development of a Live-Cell Imaging Assay to Elucidate Spatiotemporal Dynamics of Extracellular Vesicle Fusion with Target Cells - PubMed
6 hours ago
- #live-cell-imaging
- #EV-fusion
- #extracellular-vesicles
- Development of a live-cell imaging assay to study extracellular vesicle (EV) fusion with target cells.
- Use of the SunTag system for signal amplification to detect EV-binding, uptake, and fusion.
- Engineered EV-donor cells with fluorescent labels and recipient cells with anti-SunTag antibodies.
- Visualization and quantification of EV-fusion kinetics and subcellular localization.
- Potential to identify fusogenic EV subsets and molecular players modulating EV-fusion.
- Aims to enhance understanding of EV biology and improve therapeutic EV engineering.