Effect of gamma irradiation as terminal sterilization technique on the stability of oligonucleotides - PubMed
5 days ago
- #sterilization
- #oligonucleotides
- #gamma-irradiation
- Study explores gamma irradiation for terminal sterilization of oligonucleotides.
- Evaluated radioprotective factors: glycerine, histidine, trehalose, ascorbate, and dry ice.
- Homo-oligomers of deoxythymidine (5-45-mers) used as representative oligonucleotides.
- UPLC method with diode array detection quantified degradation accurately.
- Best integrity observed in samples without stabilizers at 25 kGy (5.2%-13.2% degradation).
- Histidine, trehalose, and ascorbate increased degradation (9.5%-24.2%).
- Glycerin caused extensive degradation (78.8%-96.8%).
- Dry ice during irradiation showed no protective benefit and slightly increased degradation.
- Gamma irradiation works best for oligonucleotide sterilization in aqueous solution without radioprotective factors.