Genome-wide CRISPR screens in primary human natural killer cells identify countermeasures against immunosuppressive environment - PubMed
5 hours ago
- #NK cells
- #CRISPR screens
- #immunotherapy
- CRISPR screening platform developed for primary human NK cells using BaEVRless-pseudotyped lentiviral transduction and Cas9 electroporation.
- STK17B deletion enhances NK cell expansion, while CCDC53 loss boosts degranulation and cytotoxicity.
- CRL5 complex (including RNF7, UBE2F, CISH) identified as inhibitors of IL-2 signaling and effector function under PGE2 suppression.
- Screens reveal strategies to improve NK cell persistence and efficacy in immunosuppressive tumor environments.
- Study provides scalable functional genomics platform and identifies targets for NK cell-based cancer immunotherapy.