Giant cell tumor of bone inhibits osteoblastogenesis via WNT5B - PubMed
5 hours ago
- #osteoblastogenesis
- #GCTB
- #WNT5B
- Giant cell tumor of bone (GCTB) induces overproduction of bone-resorbing osteoclasts via RANKL, leading to bone destruction.
- Denosumab, a RANKL-neutralizing antibody, is used to treat GCTB, but bone formation activity in GCTB is not well understood.
- GCTB inhibits bone formation by expressing WNT5B, which was identified through single-cell RNA sequencing and spatial transcriptome analysis.
- Co-culture of GCTB cells (GCTB1s) with human adipose-derived stem cells (ADSCs) inhibited mineralization, which was rescued by knocking out WNT5B using CRISPR/Cas9.
- ADSC supernatants were found to induce mineralization of GCTB1s.
- The study concludes that GCTB not only promotes osteoclast activity but also actively inhibits bone formation.