Structural insight into the CUB2 domain's role in enteropeptidase-mediated trypsinogen activation - PubMed
5 hours ago
- #trypsinogen activation
- #enteropeptidase
- #CUB2 domain
- The study focuses on the role of the CUB2 domain in enteropeptidase (EP)-mediated trypsinogen activation.
- Cryo-EM and enzymatic activity studies revealed the CUB2 domain's significance in cleaving macromolecular substrates.
- Key binding loops and residues crucial for EP's proteolytic function were identified.
- Mutation E574A enhanced EP's proteolytic activity, while N619A reduced cleavage efficiency, emphasizing the role of surface-charged interactions.
- A proteolytic cycle was proposed to better understand trypsinogen activation by EP.
- The findings provide insights into EP's molecular mechanisms and potential therapeutic modulation of EP-mediated proteolysis.