An in vivo CRISPR screen unveils promising target genes to improve CAR-T cell efficacy in a solid tumor model - PubMed
5 hours ago
- #CAR-T cells
- #solid tumors
- #CRISPR screen
- CAR-T cell therapies face limitations, including poor persistence and inefficacy against solid tumors.
- An in vivo CRISPR screen targeting 50 genes identified REGNASE-1, SOCS1, PTPN2, and P16INK4A as top targets to enhance CAR-T cell persistence in a lung tumor model.
- Disruption of genes like MED12, PRDM1, and BATF, previously thought beneficial, was detrimental in this context, indicating context-dependent effects.
- The study underscores the need for context-specific evaluations of genetic modifications to optimize CAR-T therapies for clinical translation.