Improving the compatibility of INFOGEST digesta with intestinal epithelial models through post-digestion storage conditions - PubMed
2 days ago
- #Food science
- #Cell model compatibility
- #In vitro digestion
- The study aimed to enhance INFOGEST digesta compatibility with intestinal epithelial models by adjusting post-digestion storage conditions.
- Five storage methods were tested: no storage, liquid nitrogen at -80°C, liquid nitrogen/freeze-drying, -20°C/freeze-drying, and -20°C.
- Condition D (-20°C/freeze-drying) significantly reduced residual trypsin activity, a key factor in epithelial cell disruption.
- This method allowed Caco-2 monolayers to maintain integrity at 25% dilution with no cytotoxicity or detachment.
- Validated with plant matrices (Brassica oleracea and Malus domestica) and in a microfluidic intestine-on-chip, showing stable TEER and ZO-1 localization.
- The optimized freeze-then-lyophilize workflow supports scalable, biocompatible integration for studying food bioaccessibility and absorption.