Phase separation of PGL-3 driven by structured domains that oligomerize and interact with RGG motifs - PubMed
5 hours ago
- #RGG motifs
- #Oligomerization
- #Phase separation
- Phase separation (PS) of PGL-3 is driven by structured domains (D1-D2) that oligomerize, not by intrinsically disordered regions (IDRs) or RNA.
- The C-terminal RGG motifs interact with D1-D2, enhancing PS even without RNA.
- The internal IDR of PGL-3 is neither necessary nor sufficient for phase separation.
- Findings challenge the assumption that PS is primarily driven by IDRs and nucleic acids.
- Study uses microscopy, crosslinking mass spectrometry, and biophysical measurements.