Spatial transcriptomics maps host-gut microbiome biogeography at high resolution - PubMed
4 days ago
- #gut microbiome
- #host-microbe interactions
- #spatial transcriptomics
- Spatial transcriptomics method maps host-gut microbiome interactions at high resolution (1 µm).
- Combines enzymatic in situ polyadenylation with spatial RNA sequencing for improved bacterial RNA recovery.
- Demonstrated improved sensitivity and resolution compared to existing workflows.
- Applied in a mouse model of intestinal neoplasia, revealing microbiome biogeography and intermicrobial interactions.
- Revealed tumour-associated changes in host-microbiome interface architecture.
- Compatible with commercial spatial RNA sequencing platforms for broad adoption.
- Patent filed by some authors on the described technology.