Sandwich ELISA using newly generated monoclonal antibodies quantifies circulating neuropeptide F in Drosophila melanogaster - PubMed
5 hours ago
- #Drosophila melanogaster
- #Neuropeptide F
- #Sandwich ELISA
- Developed a sandwich ELISA system using new monoclonal antibodies to measure circulating Neuropeptide F (NPF) in Drosophila melanogaster.
- The ELISA system has high sensitivity with a lower limit of quantification of 0.2 pM and specificity, showing no signal in NPF null mutants.
- Quantified endogenous hemolymph NPF levels in the tens of picomolar range, lower than in vitro receptor affinity, raising questions about in vivo signaling.
- Observed a modest but significant increase in circulating NPF in mated females compared to virgins, supporting previous predictions.
- Midgut-specific NPF knockdown reduced hemolymph NPF, confirming its contribution to the circulating hormone pool.