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Extrachromosomal DNA drives molecular and clinical heterogeneity in hepatocellular carcinoma: a multi-omics analysis and prognostic model development - PubMed

3 months ago
  • #Extrachromosomal DNA
  • #Prognostic model
  • #Hepatocellular carcinoma
  • Extrachromosomal DNA (ecDNA) is a key factor in cancer evolution and heterogeneity, particularly in hepatocellular carcinoma (HCC).
  • EcDNA-positive HCC tumors exhibit increased vascular invasion, higher AFP levels, and more TP53 mutations.
  • These tumors show unique activation of proliferation pathways, decreased stromal infiltration, and heightened immune activation.
  • A six-gene signature (RNF186, BMP6, AOC1, FBLL1, MYBL2, DNASE1L3) was developed for prognostic purposes.
  • DNASE1L3 was found to be downregulated in HCC and suppresses proliferation and migration of Hep3B2.1-7 cells.
  • The study provides insights into ecDNA-driven tumor heterogeneity and offers a prognostic model for personalized HCC management.