A single cluster of RNA Polymerase II molecules is stably associated with active genes - PubMed
4 hours ago
- #Transcription
- #RNA Polymerase II
- #Zygotic Genome Activation
- RNA Polymerase II (RNAPII) molecules cluster stably with active genes in eukaryotic nuclei.
- Cluster formation depends on transcription initiation, with lifetimes linked to transcriptional activity.
- Single RNAPII clusters are stably associated with active gene loci during transcription.
- Cluster intensities strongly correlate with transcriptional output.
- RNAPII clusters result from local accumulations of engaged polymerases, not higher-order mechanisms like phase separation.
- Study conducted during Zygotic Genome Activation (ZGA) in Drosophila melanogaster embryos using single-molecule tracking and lattice light-sheet microscopy.