Efficient CRISPR/Cas-SF01 genome editing tools with high editing efficiency in allotetraploid oilseed rape - PubMed
2 days ago
- #Genome Editing
- #CRISPR
- #Brassica napus
- CRISPR/Cas-SF01 is a new genome editing tool with high efficiency in allotetraploid oilseed rape.
- Cas-SF01, a Cas12i3 variant, recognizes TTN PAM sites, expanding the editable scope compared to CRISPR-Cas9.
- The study demonstrates Cas-SF01's effectiveness in protoplast and stable transformed oilseed rape lines, producing mutants with multilocular silique and male sterile phenotypes.
- Cas-SF01-derived cytosine base editors (CBEs) enable targeted C-to-T base edits.
- No off-target mutations were detected in edited plants, highlighting its precision.
- This tool enriches genome-editing resources for Brassica napus, aiding biological research and crop improvement.