Immunofluorescence Analysis for Simultaneous Liquid Biopsy of Multiple Hepatocellular Carcinoma Markers Using a Microfluidic Chip - PubMed
3 days ago
- #Liquid Biopsy
- #Microfluidic Chip
- #Cancer Diagnostics
- Developed a novel microfluidic chip, 'Pro-Exo', for simultaneous detection of multiple hepatocellular carcinoma (HCC) markers via liquid biopsy.
- Enhanced binding efficiency by using antibody-conjugated microbeads to disrupt laminar flow and enabled parallel multichannel detection.
- Integrated analysis of plasma proteins (DCP, GP73, AFP) and exosomal markers (CD9+CD81, EpCAM, GPC3) from minimal plasma volumes (50 μL per chamber).
- Achieved rapid (within 60 minutes) and sensitive detection with low limits for all biomarkers, improving early HCC identification.
- Confirmed through ROC analysis that combined biomarker detection significantly boosts diagnostic accuracy compared to single-marker approaches.