Integrated multi-omics analysis reveals a gut microbiota-tryptophan metabolism axis contributes to sex differences in a β-aminopropionitrile-induced aortic dissection mouse model - PubMed
4 hours ago
- #Aortic Dissection
- #Sex Differences
- #Tryptophan Metabolism
- Female mice were less susceptible to aortic dissection (AD) induced by β-aminopropionitrile (BAPN), showing reduced rupture rates, incidence, and aortic dilation.
- Transcriptomic analysis indicated lower inflammation-related gene induction and immune cell infiltration in female non-dissected mice.
- Metabolomic profiling revealed higher levels of tryptophan-indole pathway metabolites (e.g., indolepyruvate, indole-3-acetic acid) in female mice.
- Microbiome analysis showed greater abundance of tryptophan-metabolizing Clostridium species and upregulated genes for indolepyruvate synthesis in female mice.
- Integrated omics identified negative correlations between indole metabolites and aortic immune-inflammatory gene modules.
- In vivo experiments demonstrated that indolepyruvate treatment delayed AD progression in male mice.
- The study proposes a gut microbiota-tryptophan metabolism-aortic inflammation axis contributing to sex differences in AD.