Pre-splicing conformation and stepwise circularization of a group I intron in Azoarcus pre-tRNA - PubMed
4 hours ago
- #RNA splicing
- #Cryo-EM
- #Group I intron
- Cryo-EM structures of Azoarcus pre-tRNAIle system reveal key catalytic stages.
- Pre-splicing conformation shows a preformed P1 helix and register shifts during splicing.
- Conformational flip of G37 stabilizes the circularization site.
- Biochemical analyses confirm a two-step circularization mechanism.
- Study provides atomic-level view of group I intron splicing and circularization.
- Findings have implications for RNA conformational dynamics and ribozyme engineering.