A model for drug transport across two membranes of Gram-negative bacteria by an MFS tripartite assembly - PubMed
6 hours ago
- #drug transport
- #Gram-negative bacteria
- #MFS tripartite efflux pump
- Study presents a model for drug transport across Gram-negative bacteria's two membranes via an MFS tripartite assembly.
- Focuses on the EmrAB-TolC pump, a MFS-type tripartite efflux pump, which mediates resistance to multiple antimicrobial drugs.
- Reports the high-resolution (3.13 Å) structure of the EmrAB-TolC pump, revealing its asymmetric architecture and assembly ratio (TolC:EmrA:EmrB = 3:6:1).
- Identifies key residues involved in drug transport, supported by mutagenesis and antibiotic sensitivity assays.
- Proposes a model for one-step drug transport across the entire envelope of Gram-negative bacteria by the MFS pump.
- Highlights the importance of specialized macromolecular machines in transporting substrates across the cell envelope in Gram-negative bacteria.