A proteome-wide dependency map of protein interaction motifs - PubMed
4 days ago
- #Proteomics
- #Cell Proliferation
- #Protein Interaction
- Study focuses on Short Linear Motifs (SLiMs) in the human proteome and their role in cellular homeostasis.
- Base editing was used to mutate reported and predicted SLiMs, screening 7,293 SLiM-containing regions with 80,473 mutations in HAP1 cells.
- Identified 450 reported and 264 predicted SLiMs essential for normal cell proliferation, with reproducible results in RPE1 cells.
- Many predicted SLiMs affecting proliferation do not belong to existing classes, with binding partners identified for several, offering insights into disease-associated mutations.
- Provides a proteome-wide resource on SLiM essentiality, uncovering numerous uncharacterized essential SLiMs.