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Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse models - PubMed

5 hours ago
  • #SHANK3
  • #extracellular vesicles
  • #autism spectrum disorder
  • Extracellular vesicles (EVs) from stem cells can rescue cellular and behavioral deficits in SHANK3-associated autism spectrum disorder (ASD) models.
  • SHANK3 deficiency leads to hyperexcitability and accelerated maturation in cortical neurons, which can be transferred via EVs.
  • Proteomic analysis shows enrichment of synaptic structural regulators in SHANK3 mutant neuron-derived EVs.
  • EVs from mesenchymal stem cells (MSCs) and healthy donor iPSCs contain synaptic modulators that rescue hyperexcitability and normalize maturation in SHANK3 mutant neurons.
  • Intranasal administration of iPSC-derived EVs in Shank3B-/- mice significantly improves ASD-like behaviors, highlighting therapeutic potential.