Extraction, purification, structural elucidation, and immunomodulatory activity of Bletilla striata polysaccharides via the NF-κB signaling pathway - PubMed
8 days ago
- #Bletilla striata
- #NF-κB pathway
- #Immunomodulation
- Study focuses on extraction, purification, and structural elucidation of Bletilla striata polysaccharides (BSP-2).
- BSP-2 is a low-molecular-weight polysaccharide with immunomodulatory activity.
- Molecular weights of BSP-2 were characterized using HPGPC and NMR.
- Monosaccharide composition of BSP-2 is dominated by glucose, with minor galactose, arabinose, and rhamnose residues.
- BSP-2 inhibits pro-inflammatory mediators (IFN-γ, TNF-α, IL-1β, IL-6) in LPS-stimulated RAW264.7 cells.
- Anti-inflammatory effects of BSP-2 are mediated via the NF-κB pathway, downregulating proteins like p65, p-p65, IκBα, p-IκBα, COX-2, and iNOS.
- BSP-2 shows potential as a bioactive immunoregulatory component in functional foods and dietary supplements.