Glucose oxidase-mineralized scaffolds with multiple bioactivities for accelerated diabetic bone regeneration - PubMed
6 hours ago
- #Biomineralization
- #Bone Regeneration
- #Diabetes
- Engineered a biomimetic 3D-printed scaffold for diabetic bone regeneration.
- Integrated glucose oxidase-mineralized amorphous calcium phosphate (GOx@ACP) nanoparticles into a gelatin methacryloyl (GelMA) hydrogel.
- Biomineralization enhances enzymatic stability and enables glucose-responsive degradation.
- Dual therapeutic actions: consumes glucose to alleviate hyperglycemia and generates trace H₂O₂ to promote angiogenesis.
- Releases osteogenic ions (Ca²⁺/PO₄³⁻) upon ACP degradation.
- Sustained degradation profile ensures matched release kinetics of ions and enzymes.
- In vitro, enhances bone marrow stromal cell proliferation, osteogenic differentiation, and endothelial tubulogenesis.
- In diabetic rat cranial defects, significantly accelerated bone regeneration with bone volume (BV/TV) reaching ~52% by the 12th week.