Storage Buffer Composition Impacts Internal Structure, Freeze-Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles - PubMed
5 hours ago
- #mRNA-LNP
- #Buffer Effects
- #Freeze-Thaw Stability
- Buffer composition (Tris, histidine, citrate) affects mRNA-LNP internal structure, freeze-thaw stability, and transfection efficiency.
- Citrate buffer enhances transfection before freezing by promoting earlier HII phase transition during acidification.
- Tris buffer provides highest transfection after freeze-thaw by preventing aggregation and supporting favorable internal structure.
- Higher Tris concentrations (50-150 mM) improve freeze-thaw stability via mRNA-rich bleb formation, reducing mRNA-lipid adducts.
- Buffer influences mRNA-LNP performance by modulating internal ordering, excess lipid behavior, and endosomal escape.