β-hydroxybutyrate Modulates Neuroinflammatory Responses and Astrocyte Reactivity in an In Vitro Model of Traumatic Brain Injury - PubMed
2 days ago
- #Traumatic Brain Injury
- #Astrocyte Reactivity
- #Neuroinflammation
- β-hydroxybutyrate (BHB) from the ketogenic diet shows neuroprotective effects in a TBI in vitro model.
- BHB supplementation in neuron-glia cultures increased astrocyte density and altered morphology post-injury.
- Neuronal density was higher in BHB-supplemented cortical cultures compared to controls after injury.
- BHB reduced pro-inflammatory cytokine IL-1β and modulated GM-CSF, IFNγ, and Neuropilin-1 expression.
- GFAP expression in hippocampal cultures showed a biphasic response, indicating dynamic astrocyte regulation.
- Ultrastructural analysis suggested improved cellular integrity in BHB-supplemented cultures post-injury.
- The study provides insights into BHB's role in modulating neuroinflammatory responses and astrocyte reactivity after TBI.