LncRNA-splicing factor condensates regulate hypoxia-responsive pre-mRNA processing near nuclear speckles - PubMed
4 hours ago
- #RNA splicing
- #Hypoxia
- #Nuclear speckles
- Alternative splicing (AS) of pre-mRNA is crucial for cellular processes and linked to tumorigenesis.
- Hypoxia in malignant tumors triggers AS in numerous genes, but the mechanisms are unclear.
- Hypoxia-responsive spliced exons show inefficient splicing and are positioned near nuclear speckles.
- The lncRNA MALAT1, induced during hypoxia, associates with hypoxia-responsive genes and promotes AS.
- MALAT1 modulates the interaction between splicing factor SRSF1 and pre-mRNAs, promoting SRSF1 condensation.
- SRSF1 condensates are preferentially recruited by RNA polymerase II (RNAPII) to enhance splicing efficiency.
- MALAT1 organizes splicing factor condensates near speckles for efficient RNAPII-mediated splicing factor recruitment.